GAS CHROMATOGRAPHY IN TB DIAGNOSIS
I have a particular interest in cancer diagnosis so when I discovered a unique portable gas chromatography platform, the Znose, I was using in a cancer project, I realised that it may also have a role in TB diagnosis as a field-deployable point of care diagnostic system.
The Znose can identify bacteria without the need for derivitisation, so signatures can be library matched using live bacteria in a medium such as sputum, without first having to kill the bacteria being the normal procedure with other forms of gas chromatography.
The Znose, being a GC platform, unlike an electronic nose, is able to separate individual chemical components of bacteria that allow a more critical sample analysis to be made whilst having a resolving ceiling of pp billion where an electronic nose has a resolving ceiling of pp million; so the Znose has exquisite sensitivity.
When I introduced this platform to the London School of Hygiene, they were impressed, and we formed a collaboration which they continued to expand, leading to their publication in 2012.
The Znose is currently being used in the US to diagnose TB in conjunction with other platforms.
Collaborator: Dr Ruth McNerney.
Publication: Production of volatile organic compounds by mycobacteria. 2012
Authors: Ruth McNerney, Kim Mallard, Phyllis Ifeoma Okolo & Claire Turner.
37th World Conference Tuberculosis and Lung Disease - Paris 2006
Gino Francesco patent: A method of detecting and identifying bacteria
copyright © Gino Francesco 2013